ctcf aid egfp targeting vector pen84 Search Results


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Key resources Table
Pen84, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Addgene inc ctcf aid egfp targeting vector
Key resources Table
Ctcf Aid Egfp Targeting Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ctcf aid egfp targeting vector/product/Addgene inc
Average 92 stars, based on 1 article reviews
ctcf aid egfp targeting vector - by Bioz Stars, 2026-03
92/100 stars
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Journal: Cell

Article Title: Targeted degradation of CTCF decouples local insulation of chromosome domains from genomic compartmentalization

doi: 10.1016/j.cell.2017.05.004

Figure Lengend Snippet: Key resources Table

Article Snippet: The CTCF-AID-EGFP targeting vector (pEN84) was assembled by serial modification of the base vector pFNF (Addgene #22687) using Gibson assembly with the following templates: the minimal functional AID tag (aa 71-114) described by ( Morawska and Ulrich, 2013 ) was PCR amplified from pAID ( Nishimura et al., 2009 ); homology arms to the last exon of Ctcf were PCR amplified from E14Tg2A genomic DNA (1kb each); the N-acteyl-transferase (PAC/PuroR) was PCR amplified from pLox-STOP-Lox TOPO (Addgene # 11854), the eGFP cDNA was PCR amplified from pTRE2-2A-eGFP (Kind gift from Kevin Monahan and Stavros Lomvardas).

Techniques: Recombinant, Transfection, Software, HiChIP, In Situ